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5–4 ?mol/L variety,[156][157] the concentration of serotonin in synaptic vesicles was measured at 270 mM.[182] Following vesicular release, the resulting focus of serotonin within the synaptic cleft, to which serotonin receptors are exposed, is estimated to generally be about 300 ?mol/L. Consequently, though in vitro receptor binding affinities

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